{"created":"2023-06-20T16:39:52.076008+00:00","id":10003,"links":{},"metadata":{"_buckets":{"deposit":"92fb55ad-f7fe-4571-af95-d7988337acce"},"_deposit":{"created_by":3,"id":"10003","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"10003"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00010003","sets":["14:923:2219:2233","21:3039:3435:3437"]},"author_link":["18325","18322","18327","18321","18328","18330","18326","18329","18331","18320","18324","18323"],"item_2_alternative_title_3":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Spontaneously differentiation and gene expression analysis of trophoblast stem cells that introduces GFP gene."}]},"item_2_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-03-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"14","bibliographicPageEnd":"14","bibliographicPageStart":"9","bibliographic_titles":[{"bibliographic_title":"近畿大学先端技術総合研究所紀要"},{"bibliographic_title":"Memoirs of Institute of Advanced Technology, Kinki University","bibliographic_titleLang":"en"}]}]},"item_2_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"マウス胚盤胞期胚から栄養膜幹細胞　(trophobalst stem cells;TS細胞)が樹立された。これらはFGF4とマウス胎仔性線維芽細胞上清(MEF-CM)の存在下で未分化を維持する。さらに胚体外系譜の細胞への分化能を有する細胞である。この特徴から、胎生であり詳細な研究が困難である哺乳類における胚体外組織の発生を解明する有用な材料であると考えられている。またTS細胞は自発的分化において栄養膜巨大細胞への分化することが知られている。本実験においてGFP遺伝子を導入したTS細胞を用いることで倍数体である栄養膜巨大細胞においてGFPの蓄積量が変化すれば、形態に加えもう一つの分化の指標になるのではないかと考えられる。GFPの発現はTS細胞と同等であるかImageJを用いて蛍光強度の解析を行った。その結果、細胞の単位面積あたりの蛍光強度においては栄養膜巨大細胞へ分化することで減少する傾向が示された。しかし、細胞全体への蓄積量においてはTS細胞と比べて栄養膜巨大細胞は約70倍蓄積していたことを示した。","subitem_description_type":"Abstract"}]},"item_2_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Ota, Hiroshi","subitem_description_type":"Other"}]},"item_2_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_publisher_14":{"attribute_name":"出版者 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Satoshi"},{"subitem_text_language":"en","subitem_text_value":"Iritani, Akira"},{"subitem_text_language":"en","subitem_text_value":"Hosoi, Yoshihiko"}]},"item_2_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学生物理工学部遺伝子工学科"},{"subitem_text_value":"近畿大学大学院生物理工学研究科生物工学専攻"},{"subitem_text_value":"近畿大学大学院生物理工学研究科生物工学専攻"},{"subitem_text_value":"近畿大学大学院生物理工学研究科生物工学専攻"},{"subitem_text_value":"近畿大学大学院生物理工学研究科生物工学専攻"},{"subitem_text_value":"理化学研究所発生・再生科学総合研究センター"},{"subitem_text_value":"近畿大学生物理工学部遺伝子工学科"},{"subitem_text_value":"近畿大学生物理工学部遺伝子工学科"},{"subitem_text_value":"理化学研究所発生・再生科学総合研究センター"},{"subitem_text_value":"近畿大学生物理工学部遺伝子工学科"},{"subitem_text_value":"近畿大学生物理工学部遺伝子工学科"},{"subitem_text_value":"近畿大学生物理工学部遺伝子工学科"}]},"item_2_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Department of Genetic Engineering, Kinki University"},{"subitem_text_value":"Graduate School of Biology-Oriented Science and Technology, Kinki 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