{"created":"2023-06-20T16:39:52.011863+00:00","id":10002,"links":{},"metadata":{"_buckets":{"deposit":"d3015837-909c-40ec-b903-da950e19ed72"},"_deposit":{"created_by":3,"id":"10002","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"10002"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00010002","sets":["14:923:2219:2233","21:3039:3435:3437"]},"author_link":["18318","18313","18312","18317","18319","18315","18314","18316"],"item_2_alternative_title_3":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Study of Fertilization and Development of Wistar Rat Eggs in vitro."}]},"item_2_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-03-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"14","bibliographicPageEnd":"8","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"近畿大学先端技術総合研究所紀要"},{"bibliographic_title":"Memoirs of Institute of Advanced Technology, Kinki University","bibliographic_titleLang":"en"}]}]},"item_2_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"本研究では、Wistar系ラットを用いた体外受精ならびに、初期胚の体外培養について検討した。体外受精では、常法により過剰排卵処置を施した幼若雌ラットより卵を回収し、前培養を行うことにより、受精能を獲得させた精子と共に培養し、受精させた。その結果、Wistar (HLA) では48％ (61/127)、WistarHannover/Rccでは67％ (62/92) の排卵誘起率が得られた。続いて各種系統において、2,526個、3,238個の卵子がそれぞれ得られ、Wistar (HLA)では一匹あたり41個、WistarHannover/Rccでは一匹あたり52個の卵子が過剰排卵処置により得られた。体外受精においてWistar(HLA)では46％ (1,172/2,526)、　WistarHannover/Rccでは69％ (2,226/3,238)　の卵子が受精した。その後、77％　(900/1,172)、88％　(1,954/2,226)　の卵子がそれぞれ2細胞期胚に発生した。初期胚の体外培養では、Wistar(HLA)　では10％　(40/387)、WitarHannover/Rccでは13％　(122/923)の胚が2細胞期胚から胚盤胞期胚まで発生した。　これらの結果から、今回供試したWistar系幼若ラットは系統により差は生じるものの、過剰排卵処置および体外受精が可能であり、低率ではあるが胚盤胞期胚まで発生することが示された。","subitem_description_type":"Abstract"}]},"item_2_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学先端技術総合研究所"}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13468693","subitem_source_identifier_type":"ISSN"}]},"item_2_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Koda, Yuna"},{"subitem_text_language":"en","subitem_text_value":"Nakagawa, Takao"},{"subitem_text_language":"en","subitem_text_value":"Morita, Masahiro"},{"subitem_text_language":"en","subitem_text_value":"Ito, Kei"},{"subitem_text_language":"en","subitem_text_value":"Nagai, Tadashi"},{"subitem_text_language":"en","subitem_text_value":"Sato, Haruka"},{"subitem_text_language":"en","subitem_text_value":"Anzai, Masayuki"},{"subitem_text_language":"en","subitem_text_value":"Iritani, Akira"}]},"item_2_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学生物理工学部遺伝子工学科"},{"subitem_text_value":"(株)紀和実験動物研究所"},{"subitem_text_value":"近畿大学大学院生物理工学研究科"},{"subitem_text_value":"近畿大学生物理工学部遺伝子工学科"},{"subitem_text_value":"(株)日本医化器械製作所"},{"subitem_text_value":"近畿大学生物理工学部遺伝子工学科"},{"subitem_text_value":"近畿大学先端技術総合研究所"},{"subitem_text_value":"近畿大学先端技術総合研究所"}]},"item_2_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Department of Genetic Engineering, Kinki University"},{"subitem_text_value":"kiwa Laboratory Animal Co,Ltd."},{"subitem_text_value":"Graduate School of Biology-Oriented Science and Technology, Kinki University"},{"subitem_text_value":"Department of Genetic Engineering, Kinki University"},{"subitem_text_value":"Nihon Medical & Chemical Instruments Co, Ltd"},{"subitem_text_value":"Department of Genetic Engineering, Kinki University"},{"subitem_text_value":"Institute of Advanced Technology, Kinki University"},{"subitem_text_value":"Institute of Advanced Technology, Kinki University"}]},"item_2_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"古田,  祐奈"},{"creatorName":"コウダ, ユナ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"中川,  隆生"},{"creatorName":"ナカガワ, タカオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"森田,  真裕"},{"creatorName":"モリタ, 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