2026-03-10T19:49:15Z https://kindai.repo.nii.ac.jp/oai

oai:kindai.repo.nii.ac.jp:00013111 2023-06-20T23:18:51Z 14:923:2193:2205 21:3039:3771:3774

Biologically active substances in leaves of Fragaria×ananassa イチゴ葉に含まれる生理活性物質 大原, 祐美 オオハラ, ユミ 河越, 義晴 カワゴエ, ヨシハル 鎌田, 靖志 カマタ, ヤスシ 野崎, 勝則 ノザキ, カツノリ 森本, 正則 モリモト, マサノリ 駒井, 功一郎 コマイ, コウイチロウ [Synopsis] This paper showed that the results of isolation and identification of the biological active components in the extract obtained from strawberry leaves (Fragaria × ananassa cv. Ama-Ou) after harvesting of their fruits. The tyrosinase inhibition was evaluated for potential human skin whitening, and super oxide dismutase (SOD) mimic activity was evaluated for potential antioxidation in the human body. Moreover, hyaluronidase inhibition, a—glucosidase inhibition and melanin biosynthesis inhibition test used by using B16 mouse melanoma cell line, were carried out evaluated in this experiment. The 50％ ethanol extract from strawberry leaves showed both of tyrosinase and hyaluronidase inhibition, and SOD mimic activity. Following active compounds were isolated by silica gel column chromatography and HPLC using tyrosinase inhibitory activity bioassay guided fractionation. These active compounds identified the tannin, agrimoniin and flavonoid glucosides, quercetin-3—O—D—glucopyranoside, quercetin-3—O—disaccharide, but this compound was not totally elucidated the chemical structure. Though, three isolated active ingredients in the 50％ ethanol extract, did not show the cell propagation activity against NB1RGB cell line, all compounds showed both of antioxidant and melanin biosynthetic inhibitory activity in this study. Especially, agrimoniin was a strong tyrosinase and hyaluronidase inhibitor in them. These facts suggested that the strawberry leaves 50％ ethanol extract was utilized as food adductand cosmetic material from natural resources. application/pdf departmental bulletin paper 近畿大学資源再生研究所 2009-03-01 VoR application/pdf 近畿大学資源再生研究所報告 7 45 55 Annual report of the institute of resource recycling of Kinki University https://kindai.repo.nii.ac.jp/record/13111/files/AA1247496X-20090331-0045.pdf https://kindai.repo.nii.ac.jp/records/13111 jpn