2026-03-09T02:59:34Z https://kindai.repo.nii.ac.jp/oai

oai:kindai.repo.nii.ac.jp:02002525 2025-03-05T04:48:03Z 14:923:1379:1740538581317

エイコサペンタエン酸は骨格筋の遺伝子発現を変化させ筋クランプ(足のつり)発症を予防する Eicosapentaenoic acid alters gene expression profiles in the skeletal muscle and prevents the incidence of muscle cramps in mouse 有本, 明寿香 ARIMOTO, Asuka 近畿大学大学院 Graduate School of Kinki University 井通, 美祈 ITUU, Minori 近畿大学 Kindai University 堀井, 鴻佑 HORII, Kosuke 近畿大学大学院 Graduate School of Kinki University 森島, 真幸 MORISHIMA, Masaki 近畿大学 Kindai University muscle cramp eicosapentaenoic acid metabolic acidosis Muscle cramps are painful, sudden and involuntary muscle contractions that are generally self-limiting. However, the underlying pathophysiological mechanisms have not been fully elucidated. The objective of this study was to establish a muscle cramp-induced mouse model and to investigate the preventive effect of eicosapentaenoic acid (EPA) on muscle cramps. Male C57BL/6 mice were fed a normal diet with sesame oil, with or without EPA, for six weeks. After four weeks, the muscle cramp group was treated with a vasopressin V₂ receptor antagonist and ammonium chloride. Metabolic acidosis was confirmed by a significant reduction in serum pH and an increase in AQP2 mRNA expression in the kidney of the cramp group. In contrast, EPA administration prevented the decrease in serum pH, increased Akt phosphorylation and shifted MyHC composition toward the slow-twitch muscle fiber type in the soleus muscle. These results suggest that this muscle cramp model may serve as a valuable experimental tool. Furthermore, they indicate that EPA administration may prevent muscle cramps by activating signaling pathways that promote protein synthesis, specifically in slow-twitch muscle fibers. 近畿大学農学部 2025-03-31 jpn departmental bulletin paper AM https://kindai.repo.nii.ac.jp/records/2002525 21896267 近畿大学農学部紀要 MEMOIRS OF THE FACULTY OF AGRICULTURE OF KINDAI UNIVERSITY 58 44 57 https://kindai.repo.nii.ac.jp/record/2002525/files/AN00064044-20250331-0044.pdf application/pdf 693.1 KB