2026-03-14T06:08:18Z https://kindai.repo.nii.ac.jp/oai

oai:kindai.repo.nii.ac.jp:00013885 2023-06-20T23:08:18Z 14:923:2261:2262 21:3039:3842:3848

〈原著論文〉UCST型ノニオン系ポリマーを表面修飾したガラス基板の調製とHeLa細胞の温度刺激はく離 Preparation of UCST types of nonion polymer modified glass and thermal stimuli-exfoliation of HeLa cells on the substrate 今城, 明典 イマジョウ, アキノリ 伊藤, 大時 イトウ, タイジ 山田, 康枝 ヤマダ, ヤスエ 白石, 浩平 シライシ, コウヘイ Poly(acrylamide-co-acrylonitrile) Upper critical solution temperature (UCST) Surface initiated atom transfer radical polymerization(SI-ATRP) Thermal exfoliation HeLa cells Poly (acrylamide-co-acrylonitrile)[poly (AAm-co-AN)] with upper critical solution temperature (UCST) was grafted onto a glass substrate by surface initiated atom transfer radical polymerization(SI-ATRP). From the scanning probe microscope(SPM) measurements, poly-(AAm-co-AN) brushes were observed on the glass substrate(g-glass). The contact angle(θ) value of g-glass to water at 26℃ increased from θ=21°(the untreated glass) to θ=69°. On the other hand, that of g-glass at 40℃ decreased from θ=69°to θ=65°.　After being HeLa cells attached and proliferated on the g-glass at 30℃ under UCST of poly(AAm-co-AN) burshes, the HeLa cells attached were thermally exfoliated by increasing culture temperature over 37℃ for 30 min in phosphate buffered saline(pH7.4). The % of mechanical and thermal stimuli-exfoliation　of attached HeLa cells on the g-glass was about 90% despite of about 10% on untreated glass. Ⅲ.論文集 application/pdf 近畿大学次世代基盤技術研究所 2015-06-01 jpn departmental bulletin paper VoR https://kindai.repo.nii.ac.jp/records/13885 21858802 近畿大学次世代基盤技術研究所報告 Kinki University Research Institute of Fundamental Technology for Next Generation 6 55 59 https://kindai.repo.nii.ac.jp/record/13885/files/AA12517632-20150600-0055.pdf application/pdf 1.5 MB 2016-02-19