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パーティクルガン法を利用したオオムギ子葉鞘細胞およびオオムギうどんこ病菌への外来遺伝子の導入
https://kindai.repo.nii.ac.jp/records/9859
https://kindai.repo.nii.ac.jp/records/98598b96827e-b4f2-4fd2-901b-54563af39796
名前 / ファイル | ライセンス | アクション |
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AN10410378-20000315-0089.pdf (491.3 kB)
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Item type | ☆紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2011-11-22 | |||||
タイトル | ||||||
タイトル | パーティクルガン法を利用したオオムギ子葉鞘細胞およびオオムギうどんこ病菌への外来遺伝子の導入 | |||||
その他(別言語等)のタイトル | ||||||
その他のタイトル | Transformation of barley coleoptile cells and powdery mildew by microprojectile bombardment | |||||
著者 |
松田, 克礼
× 松田, 克礼× 豊田, 秀吉× 倉田, 浩子× 瀧川, 義浩× 野々村, 照雄× 大内, 成志 |
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言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者(英) | ||||||
en | ||||||
Matsuda, Yoshinori | ||||||
著者(英) | ||||||
en | ||||||
Toyoda, Hideyoshi | ||||||
著者(英) | ||||||
en | ||||||
Kurata, Hiroko | ||||||
著者(英) | ||||||
en | ||||||
Takikawa, Yoshihiro | ||||||
著者(英) | ||||||
en | ||||||
Nonomura, Teruo | ||||||
著者(英) | ||||||
en | ||||||
Ouchi, Seiji | ||||||
著者 所属 | ||||||
近畿大学農学総合研究所 | ||||||
著者 所属 | ||||||
近畿大学農学部農学科 | ||||||
著者 所属 | ||||||
近畿大学農学部農学科 | ||||||
著者 所属 | ||||||
近畿大学農学部農学科 | ||||||
著者 所属 | ||||||
近畿大学農学部農学科 | ||||||
著者 所属 | ||||||
近畿大学農学部農学科:近畿大学農学総合研究所 | ||||||
著者所属(翻訳) | ||||||
Institute for Comprehensive Agricultural Sciences, Kinki University | ||||||
著者所属(翻訳) | ||||||
Department of Agronomy, Faculty of Agriculture, Kinki University | ||||||
著者所属(翻訳) | ||||||
Department of Agronomy, Faculty of Agriculture, Kinki University | ||||||
著者所属(翻訳) | ||||||
Department of Agronomy, Faculty of Agriculture, Kinki University | ||||||
著者所属(翻訳) | ||||||
Department of Agronomy, Faculty of Agriculture, Kinki University | ||||||
著者所属(翻訳) | ||||||
Department of Agronomy, Faculty of Agriculture, Kinki University:Institute for Comprehensive Agricultural Sciences, Kinki University | ||||||
版 | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 名前 | ||||||
出版者 | 近畿大学農学総合研究所 | |||||
書誌情報 |
近畿大学農学総合研究所報告 en : Bulletin of the Institute for Comprehensive Agricultural Sciences, Kinki University 号 8, p. 89-94, 発行日 2000-03-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09193022 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | [Synopsis] In this study, we established a gene delivery system for Biumeria graminis f. sp. hordei by microprojectile bombardment. Tungsten particles were coated with the plasmid pBI/s-GFP which had been constructed from pBI121 by replacing the GUS gene with s-GFP. The coated plasmid was introduced into barley coleoptile epidermal cells or conidiospores of B. graminis f. sp. hordei by microprojectile bombardment. The expression of s-GFP gene in transformed coleoptile cells and conidiospores were detected by green fluorescence of GFP under the fluorescent microscope. The fluorescent condiospores were transferred by micromanipulation to non-inoculated coleoptile tissues to differentiated from non-transformed conidiospores. The transferred conidiospores germinated, invaded after appressorium formation, formed haustria and secondary hyphae, indicating that the gene delivery system established in this study is useful for introducing foreign genes not only into barley coleoptile epidermal cells and B. graminis f. sp. hordei, but also other obligately parasitic disease complexes, because easy detection of introduced gene expression in the transformed cells. | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf |