WEKO3
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ユキエ", "creatorNameLang": "ja-Kana"}], "nameIdentifiers": [{"nameIdentifier": "8756", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "竹下, 由美"}, {"creatorName": "タケシタ, ユミ", "creatorNameLang": "ja-Kana"}], "nameIdentifiers": [{"nameIdentifier": "8757", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "土山, 修治"}, {"creatorName": "ツチヤマ, シュウジ", "creatorNameLang": "ja-Kana"}], "nameIdentifiers": [{"nameIdentifier": "8758", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "石川, 裕子"}, {"creatorName": "イシカワ, ユウコ", "creatorNameLang": "ja-Kana"}], "nameIdentifiers": [{"nameIdentifier": "8759", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "石束, 祐太"}, {"creatorName": "イシズカ, ユウタ", "creatorNameLang": "ja-Kana"}], "nameIdentifiers": [{"nameIdentifier": "8760", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "細井, 美彦"}, {"creatorName": "ホソイ, ヨシヒコ", "creatorNameLang": "ja-Kana"}], "nameIdentifiers": 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〈Original Papers〉マウス体外成熟由来2細胞期胚を用いた冷蔵輸送・保存方法の検討
https://kindai.repo.nii.ac.jp/records/5754
https://kindai.repo.nii.ac.jp/records/5754a4ab9a77-3e94-414a-8166-af775bdd6157
名前 / ファイル | ライセンス | アクション |
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AA11153712-20120331-0033.pdf (1.5 MB)
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Item type | ☆紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2012-04-16 | |||||
タイトル | ||||||
タイトル | 〈Original Papers〉マウス体外成熟由来2細胞期胚を用いた冷蔵輸送・保存方法の検討 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | 〈Original Papers〉Investigation of transport and preservation at cool temperature using mice 2-cell stage embryos produced by in vitro maturation | |||||
著者 |
西村, 愛美
× 西村, 愛美× 中牟田, 裕子× 福本, 紀代子× 近藤, 朋子× 春口, 幸恵× 竹下, 由美× 土山, 修治× 石川, 裕子× 石束, 祐太× 細井, 美彦× 三谷, 匡× 竹尾, 透× 中潟, 直己× 安齋, 政幸 |
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言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者(英) | ||||||
en | ||||||
Nishimura, Manami | ||||||
著者(英) | ||||||
en | ||||||
Nakamuta, Yuko | ||||||
著者(英) | ||||||
en | ||||||
Fukumoto, Kiyoko | ||||||
著者(英) | ||||||
en | ||||||
Kondo, Tomoko | ||||||
著者(英) | ||||||
en | ||||||
Haruguchi, Yukie | ||||||
著者(英) | ||||||
en | ||||||
Takeshita, Yumi | ||||||
著者(英) | ||||||
en | ||||||
Tsuchiyama, Shuji | ||||||
著者(英) | ||||||
en | ||||||
Ishikawa, Yuko | ||||||
著者(英) | ||||||
en | ||||||
Ishizuka, Yuta | ||||||
著者(英) | ||||||
en | ||||||
Hosoi, Yoshihiko | ||||||
著者(英) | ||||||
en | ||||||
Mitani, Tasuku | ||||||
著者(英) | ||||||
en | ||||||
Takeo, Toru | ||||||
著者(英) | ||||||
en | ||||||
Nakagata, Naomi | ||||||
著者(英) | ||||||
en | ||||||
Anzai, Masayuki | ||||||
著者 所属 | ||||||
近畿大学大学院生物理工学研究科生物工学専攻 | ||||||
著者 所属 | ||||||
熊本大学生命資源研究・支援センター動物資源開発部門(CARD):九動株式会社 | ||||||
著者 所属 | ||||||
熊本大学生命資源研究・支援センター動物資源開発部門(CARD):九動株式会社 | ||||||
著者 所属 | ||||||
熊本大学生命資源研究・支援センター動物資源開発部門(CARD):九動株式会社 | ||||||
著者 所属 | ||||||
熊本大学生命資源研究・支援センター動物資源開発部門(CARD):九動株式会社 | ||||||
著者 所属 | ||||||
熊本大学生命資源研究・支援センター動物資源開発部門(CARD):九動株式会社 | ||||||
著者 所属 | ||||||
熊本大学生命資源研究・支援センター動物資源開発部門(CARD) | ||||||
著者 所属 | ||||||
近畿大学生物理工学部遺伝子工学科 | ||||||
著者 所属 | ||||||
近畿大学生物理工学部遺伝子工学科 | ||||||
著者 所属 | ||||||
近畿大学大学院生物理工学研究科生物工学専攻:近畿大学生物理工学部遺伝子工学科:近畿大学先端技術総合研究所 | ||||||
著者 所属 | ||||||
近畿大学先端技術総合研究所 | ||||||
著者 所属 | ||||||
熊本大学生命資源研究・支援センター動物資源開発部門(CARD) | ||||||
著者 所属 | ||||||
熊本大学生命資源研究・支援センター動物資源開発部門(CARD) | ||||||
著者 所属 | ||||||
近畿大学先端技術総合研究所 | ||||||
著者所属(翻訳) | ||||||
Division of Biologica Science, Graduate School of Biology-Oriented Science and Technology, Kinki University | ||||||
著者所属(翻訳) | ||||||
Division of Reproductive Engineering, Center for Animal Resources and Development (CARD):Kyudo Co., Ltd | ||||||
著者所属(翻訳) | ||||||
Division of Reproductive Engineering, Center for Animal Resources and Development (CARD):Kyudo Co., Ltd | ||||||
著者所属(翻訳) | ||||||
Division of Reproductive Engineering, Center for Animal Resources and Development (CARD):Kyudo Co., Ltd | ||||||
著者所属(翻訳) | ||||||
Division of Reproductive Engineering, Center for Animal Resources and Development (CARD):Kyudo Co., Ltd | ||||||
著者所属(翻訳) | ||||||
Division of Reproductive Engineering, Center for Animal Resources and Development (CARD):Kyudo Co., Ltd | ||||||
著者所属(翻訳) | ||||||
Division of Reproductive Engineering, Center for Animal Resources and Development (CARD) | ||||||
著者所属(翻訳) | ||||||
Development of Biology-Oriented Science and Technology, Kinki University | ||||||
著者所属(翻訳) | ||||||
Development of Biology-Oriented Science and Technology, Kinki University | ||||||
著者所属(翻訳) | ||||||
Division of Biologica Science, Graduate School of Biology-Oriented Science and Technology, Kinki University:Development of Biology-Oriented Science and Technology, Kinki University:Institute of Advanced Technology, Kinki University | ||||||
著者所属(翻訳) | ||||||
Institute of Advanced Technology, Kinki University | ||||||
著者所属(翻訳) | ||||||
Division of Reproductive Engineering, Center for Animal Resources and Development (CARD) | ||||||
著者所属(翻訳) | ||||||
Division of Reproductive Engineering, Center for Animal Resources and Development (CARD) | ||||||
著者所属(翻訳) | ||||||
Institute of Advanced Technology, Kinki University | ||||||
版 | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 名前 | ||||||
出版者 | 近畿大学生物理工学部 | |||||
書誌情報 |
Memoirs of the Faculty of Biology-Oriented Science and Technology of Kinki University en : 近畿大学 生物理工学部 紀要 号 29, p. 33-41, 発行日 2012-03-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13427202 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | [要旨] 遺伝資源としての生殖細胞の保存および輸送方法の確立のために、従来の超低温下による凍結保存に変わる技術として、新たに私たちは初期胚の低温保存および冷蔵輸送方法を開発した。本研究では、排卵に至らない卵巣内に存在する未成熟卵子を用いて得られた体外受精由来2細胞期胚の冷蔵輸送方法および産子への発生能を検討した。成熟マウス卵巣内よりGV期卵子を回収し体外成熟後に発生したMH期卵子(1,72211,920:90%)を透明帯穿孔処理により体外受精をおこなったところ56%(5521992)の受精成績であり、83%(4571552)が2細胞期胚へと発生した。これらの胚は、近畿大学先端技術総合研究所(和歌山)から熊本大学・CARD(熊本)冷蔵輸送したところ、約48時間後における冷蔵保存胚は、100%(2401240)の生存成績であり、それらの一部の胚を移植した結果、10%(211213)の産子を得た。以上の結果より、体外成熟由来2細胞期胚の冷蔵輸送・冷蔵保存が可能であることが示された。 [Abstract] We developed transport and preservation at cool temperature of early embryo for substitution of traditional vitrification technique to establish preservation and transport method of Bio Resource as for gamete. This study was investigation of transport and preservation at cool temperature and development of live youngs using mice 2-cell stage produced by in vitro matured oocytes. In vitro matured oocytes (90%:1,722/1,920) obtained from C57BL/6J ovary were fertilized using laser optimal system (56%: 552/992). Fertilized eggs were developed to 2-cell stage (83%457/552). Sampling tubes containing 2-cell stage embryos produced by in vitro fertilization were send to the Center for Animal Resources and Development (CARD) , Kumamoto University, Kumamoto from the Institute of Advanced Technology, Kinki University, Wakayama. At CARD, the embryos were recovered from the sampling tubes. The rate of viable 2-cell stage embryos after transport was 100% (240/240). Collected 2-cell stage embryos were transferred into the oviducts of pseudopregnant females on the day that a vaginal plug was found (Day 1 of pseudopregnancy). Two cell stage embryos transferred to oviducts were developed to live youngs (10%: 21/213). | |||||
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内容記述タイプ | Other | |||||
内容記述 | application/pdf |