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イチゴ品種宝交早生の葯培養による幼苗生産
https://kindai.repo.nii.ac.jp/records/4968
https://kindai.repo.nii.ac.jp/records/496853d79312-b64a-412c-9160-d2cfece30166
名前 / ファイル | ライセンス | アクション |
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AN00064044-19910315-0037.pdf (1.3 MB)
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Item type | ☆紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2008-01-28 | |||||
タイトル | ||||||
タイトル | イチゴ品種宝交早生の葯培養による幼苗生産 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Production of Young Plants by Anther Culture of Strawberry, variety Houkouwase | |||||
著者 |
衣川, 堅二郎
× 衣川, 堅二郎× 種坂, 英次× 芝尾, 健 |
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言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者(英) | ||||||
en | ||||||
Kinugawa, Kenjiro | ||||||
著者(英) | ||||||
en | ||||||
Tanesaka, Eiji | ||||||
著者(英) | ||||||
en | ||||||
Shibao, Ken | ||||||
著者 所属 | ||||||
近畿大学農学部農学科遺伝育種学研究室 | ||||||
著者 所属 | ||||||
近畿大学農学部農学科遺伝育種学研究室 | ||||||
著者 所属 | ||||||
近畿大学農学部農学科遺伝育種学研究室 | ||||||
著者所属(翻訳) | ||||||
Laboratory of Genetics and Plant Breeding, Department of Agriculture, Faculty of Agriculture, Kinki University | ||||||
著者所属(翻訳) | ||||||
Laboratory of Genetics and Plant Breeding, Department of Agriculture, Faculty of Agriculture, Kinki University | ||||||
著者所属(翻訳) | ||||||
Laboratory of Genetics and Plant Breeding, Department of Agriculture, Faculty of Agriculture, Kinki University | ||||||
版 | ||||||
出版タイプ | NA | |||||
出版タイプResource | http://purl.org/coar/version/c_be7fb7dd8ff6fe43 | |||||
出版者 名前 | ||||||
出版者 | 近畿大学農学部 | |||||
書誌情報 |
近畿大学農学部紀要 en : Memoirs of the Faculty of Agriculture of Kinki University 号 24, p. 37-45, 発行日 1991-01-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 04538889 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | [Author abstract]Culture conditions of anther culture of strawberry, variety Houkouwase, were studied to improve conventional mass production technology of young plants by callus. Anthers about 1 mm long easily produced callus on the MS and LS media supplemented with sucrose (30 g/l), agar (8 g/l), NAA (0.2-2.0 mg/l), and BA (0.2-2.0 mg/l). Presence of anthers of appropriate size for callus induction inside bud was estimated by the size of the bud and by the length of the bud stalk. Enough aeration and supply of fresh medium to calli seemed to accerelate their propagation in culture. Plant-regeneration was enhanced by transplanting growing calli from the medium with NAA of low concentration onto the medium containing 0.2 mg/l NAA and 2.0 mg/l BA. A practical handling method to produce young plants was also presented.[著者抄録]イチゴ品種宝交早生を用い,ウイルスフリー苗の簡便な大量培養法を開発するための実験を行った.葯を無ウイルス組織として用い,カルス化から植物体再生をへて幼苗生産までの培養諸条件を検討した.カルス化率の高い約は長さ1mm前後が適当で,MS又はLS培地に薦糖30g/l,寒天8g/l,NAA 0.2~2.0mg/l,BA 0.2~2.0mg/lを添加した培地上でよくカルス化し,増殖した.また,培地にNAAまたはBAのどちらかが欠けても,カルス化もカルス増殖もおこらなかった.通気と新鮮培地の不断の補給はカルス増殖を著しく促進すると推定された.カルス塊をNAA 0.2mg/l+BA 2.0mg/lで増殖し,次に同一組成の培地へ移殖し培養を継続したとき,植物体再生が最も早く豊富に生じた.これらの知見をもとに,葯培養に始る幼苗育成工程を試作した. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 記事区分:資料 | |||||
サムネイル |
AN00064044-19910315-0037.jpeg |
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フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf |