WEKO3
アイテム
{"_buckets": {"deposit": "803c3261-e01f-49ba-b7c1-d66a9c260607"}, "_deposit": {"created_by": 3, "id": "11623", "owners": [3], "pid": {"revision_id": 0, "type": "depid", "value": "11623"}, "status": "published"}, "_oai": {"id": "oai:kindai.repo.nii.ac.jp:00011623", "sets": ["3624"]}, "author_link": ["22351", "22352", "22353"], "control_number": "11623", "item_2_biblio_info_21": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2015-12-01", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "23", "bibliographicPageEnd": "4", "bibliographicPageStart": "1", "bibliographic_titles": [{"bibliographic_title": "かやのもり:近畿大学産業理工学部研究報告", "bibliographic_titleLang": "ja"}, {"bibliographic_title": "Reports of Faculty of Humanity-Oriented Science and Engineering, Kinki University", "bibliographic_titleLang": "en"}]}]}, "item_2_date_19": {"attribute_name": "日付 作成日", "attribute_value_mlt": [{"subitem_date_issued_datetime": "2015-12-16", "subitem_date_issued_type": "Created"}]}, "item_2_description_33": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "In the present study, we investigated the intracellular delivery of siRNA using synthetic peptides as transfection reagents and the silencing effect of siRNA targeting hTERT mRNA in 3 human cancer cell lines, Jurkat, HeLa and K562. The complex of siRNA and a specific amphiphilic peptide could be effectively taken up into cells. The complex also showed a high silencing effect against hTERT mRNA. Moreover, the combination of siRNA-NES conjugates and the amphiphilic peptides improved silencing effects up to 95.2%. The amphiphilic peptides and their hybrids showed almost no cyto-toxicity and protected siRNA against intracellular nuclease digestion in 10% FBS (half life time was over 48h).", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_2_description_37": {"attribute_name": "資源タイプ(WEKO2)", "attribute_value_mlt": [{"subitem_description": "Departmental Bulletin Paper", "subitem_description_type": "Other"}]}, "item_2_description_41": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_2_identifier_registration": {"attribute_name": "ID登録", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.15100/0000011623", "subitem_identifier_reg_type": "JaLC"}]}, "item_2_publisher_14": {"attribute_name": "出版者 名前", "attribute_value_mlt": [{"subitem_publisher": "近畿大学産業理工学部"}]}, "item_2_source_id_22": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "13495801", "subitem_source_identifier_type": "ISSN"}]}, "item_2_text_15": {"attribute_name": "出版社 カナ", "attribute_value_mlt": [{"subitem_text_value": "キンキ ダイガク サンギョウリコウ ガクブ"}]}, "item_2_text_16": {"attribute_name": "出版社 ローマ字", "attribute_value_mlt": [{"subitem_text_value": "Kinki daigaku sangyoriko gakubu"}]}, "item_2_text_17": {"attribute_name": "出版年(from)", "attribute_value_mlt": [{"subitem_text_value": "2015"}]}, "item_2_text_18": {"attribute_name": "出版年(to)", "attribute_value_mlt": [{"subitem_text_value": "12-10"}]}, "item_2_text_44": {"attribute_name": "ID(XooNIps)", "attribute_value_mlt": [{"subitem_text_value": "AA12013209-20151210-0001"}]}, "item_2_text_47": {"attribute_name": "日付 記録日(XooNIps)", "attribute_value_mlt": [{"subitem_text_value": "2015-12-16"}]}, "item_2_text_51": {"attribute_name": "最終更新日(XooNIps)", "attribute_value_mlt": [{"subitem_text_value": "Dec 22, 2015 09:00:00"}]}, "item_2_text_53": {"attribute_name": "登録者(XooNIps)", "attribute_value_mlt": [{"subitem_text_value": "kurepo"}]}, "item_2_text_54": {"attribute_name": "閲覧数(XooNIps)", "attribute_value_mlt": [{"subitem_text_value": "90"}]}, "item_2_text_55": {"attribute_name": "ダウンロード数(XooNIps)", "attribute_value_mlt": [{"subitem_text_value": "73"}]}, "item_2_text_56": {"attribute_name": "XooNIps_ITEM_KEY", "attribute_value_mlt": [{"subitem_text_value": "18645"}]}, "item_2_text_8": {"attribute_name": "著者 所属", "attribute_value_mlt": [{"subitem_text_value": "近畿大学産業理工学部生物環境化学科"}, {"subitem_text_value": "近畿大学産業理工学部生物環境化学科"}, {"subitem_text_value": "近畿大学産業理工学部生物環境化学科; 教授"}]}, "item_2_version_type_12": {"attribute_name": "版", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorAffiliations": [{"affiliationNames": [{"affiliationName": "近畿大学", "affiliationNameLang": "ja"}, {"affiliationName": "Kinki University", "affiliationNameLang": "en"}]}], "creatorNames": [{"creatorName": "新貝, 恭広", "creatorNameLang": "ja"}, {"creatorName": "シンカイ, ヤスヒロ", "creatorNameLang": "ja-Kana"}, {"creatorName": "Shinkai, Yasuhiro", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "22351", "nameIdentifierScheme": "WEKO"}]}, {"creatorAffiliations": [{"affiliationNames": [{"affiliationName": "近畿大学", "affiliationNameLang": "ja"}, {"affiliationName": "Kinki University", "affiliationNameLang": "en"}]}], "creatorNames": [{"creatorName": "柏原, 慎一", "creatorNameLang": "ja"}, {"creatorName": "カシハラ, シンイチ", "creatorNameLang": "ja-Kana"}, {"creatorName": "Kashihara, Shinichi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "22352", "nameIdentifierScheme": "WEKO"}]}, {"creatorAffiliations": [{"affiliationNames": [{"affiliationName": "近畿大学", "affiliationNameLang": "ja"}, {"affiliationName": "Kinki University", "affiliationNameLang": "en"}]}], "creatorNames": [{"creatorName": "藤井, 政幸", "creatorNameLang": "ja"}, {"creatorName": "フジイ, マサユキ", "creatorNameLang": "ja-Kana"}, {"creatorName": "Fujii, Masayuki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "22353", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-02-19"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "AA12013209-20151210-0001.pdf", "filesize": [{"value": "1.4 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1400000.0, "url": {"label": "AA12013209-20151210-0001.pdf", "url": "https://kindai.repo.nii.ac.jp/record/11623/files/AA12013209-20151210-0001.pdf"}, "version_id": "6f57e41f-99ff-48db-88fd-3f1fd0fbe0cb"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "siRNA", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "transfection", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "synthetic peptide", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "gene silencing", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "〈論文・報告〉新規合成ペプチドによるsiRNAの無毒性細胞導入と遺伝子サイレンシング", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "〈論文・報告〉新規合成ペプチドによるsiRNAの無毒性細胞導入と遺伝子サイレンシング", "subitem_title_language": "ja"}, {"subitem_title": "〈PAPERS and REPORTS〉Non-Toxic Transfection of siRNA by Novel Synthetic Peptides and Gene Silencing", "subitem_title_language": "en"}]}, "item_type_id": "2", "owner": "3", "path": ["3624", "3625"], "permalink_uri": "https://doi.org/10.15100/0000011623", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2015-12-22"}, "publish_date": "2015-12-22", "publish_status": "0", "recid": "11623", "relation": {}, "relation_version_is_last": true, "title": ["〈論文・報告〉新規合成ペプチドによるsiRNAの無毒性細胞導入と遺伝子サイレンシング"], "weko_shared_id": -1}
〈論文・報告〉新規合成ペプチドによるsiRNAの無毒性細胞導入と遺伝子サイレンシング
https://doi.org/10.15100/0000011623
https://doi.org/10.15100/00000116235633e6fb-164d-4adc-8ab0-785ec586d8fd
名前 / ファイル | ライセンス | アクション |
---|---|---|
AA12013209-20151210-0001.pdf (1.4 MB)
|
|
Item type | ☆紀要論文 / Departmental Bulletin Paper(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2015-12-22 | |||||
タイトル | ||||||
言語 | ja | |||||
タイトル | 〈論文・報告〉新規合成ペプチドによるsiRNAの無毒性細胞導入と遺伝子サイレンシング | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | 〈PAPERS and REPORTS〉Non-Toxic Transfection of siRNA by Novel Synthetic Peptides and Gene Silencing | |||||
著者 |
新貝, 恭広
× 新貝, 恭広× 柏原, 慎一× 藤井, 政幸 |
|||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題 | siRNA, transfection, synthetic peptide, gene silencing | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ID登録 | ||||||
ID登録 | 10.15100/0000011623 | |||||
ID登録タイプ | JaLC | |||||
著者 所属 | ||||||
近畿大学産業理工学部生物環境化学科 | ||||||
著者 所属 | ||||||
近畿大学産業理工学部生物環境化学科 | ||||||
著者 所属 | ||||||
近畿大学産業理工学部生物環境化学科; 教授 | ||||||
版 | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 名前 | ||||||
出版者 | 近畿大学産業理工学部 | |||||
書誌情報 |
ja : かやのもり:近畿大学産業理工学部研究報告 en : Reports of Faculty of Humanity-Oriented Science and Engineering, Kinki University 号 23, p. 1-4, 発行日 2015-12-01 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13495801 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | In the present study, we investigated the intracellular delivery of siRNA using synthetic peptides as transfection reagents and the silencing effect of siRNA targeting hTERT mRNA in 3 human cancer cell lines, Jurkat, HeLa and K562. The complex of siRNA and a specific amphiphilic peptide could be effectively taken up into cells. The complex also showed a high silencing effect against hTERT mRNA. Moreover, the combination of siRNA-NES conjugates and the amphiphilic peptides improved silencing effects up to 95.2%. The amphiphilic peptides and their hybrids showed almost no cyto-toxicity and protected siRNA against intracellular nuclease digestion in 10% FBS (half life time was over 48h). | |||||
言語 | en | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf |