Kindai University
Kindai University
Kindai University
Kindai University
Kindai University
Kindai University
Kindai University
Kindai University
版
Pubisher
出版者 名前
近畿大学産業理工学部
出版社 カナ
キンキ ダイガク サンギョウリコウ ガクブ
出版社 ローマ字
Kinki daigaku sangyoriko gakubu
出版年(from)
2016
出版年(to)
12-15
日付 作成日
2017-01-13
雑誌名
かやのもり：近畿大学産業理工学部研究報告
雑誌名(英)
Reports of Faculty of Humanity-Oriented Science and Engineering, Kindai University
号
25
ページ
1 - 6
発行年
2016-12-15
ISSN
13495801
抄録
RNA interference (RNAi) is a genetic regulation system induced by small RNAs. There are two pathways in RNAi, one is induced by small interfering RNA (siRNA) and the other by micro RNA (miRNA). siRNA is a full matched 21-23nt dsRNA and thought to evolutionally develop as a biological defense mechanism against RNA viruses. On the other hand, miRNA is an endogenously transcribed 20-25nt dsRNA having two or three mismatches and is involved in various biological processes. Based on structural and mechanistic similarities and differences between siRNA and miRNA, RNAi efficiencies of siRNAs with 3 mismatches at 2, 10 and 14 positions and siRNAs with 2 mismatches at 2 and 14 positions were evaluated. As a result, siRNA bearing a mismatch at central position showed weaker RNAi effect than siRNAs with no mismatch at central position. It can be concluded that RNAi by Ago2-RISC is stronger than that by Ago1-RISC.
〈論文・報告〉miRNA様siRNAによる遺伝子サイレンシング効果
AA12013209-20161215-0001.pdf
(1.02MB)
