WEKO3
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〈論文・報告〉化学修飾small interfering RNA (siRNA)による遺伝子サイレンシング
https://doi.org/10.15100/0000017669
https://doi.org/10.15100/0000017669f563876c-901c-4009-bdf8-dc66022dc1f1
名前 / ファイル | ライセンス | アクション |
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AA12013209-20160715-0001.pdf (1.3 MB)
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Item type | ☆紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2016-08-03 | |||||
タイトル | ||||||
言語 | ja | |||||
タイトル | 〈論文・報告〉化学修飾small interfering RNA (siRNA)による遺伝子サイレンシング | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Gene Silencing by Chemically Modified Small Interfering RNA(siRNA) | |||||
著者 |
新貝, 恭広
× 新貝, 恭広× 柏原, 慎一× 藤井, 啓史× 峰松, 剛× 吉永, 尚平× 苗村, 円佳× 神武, 洋二郎× 藤井, 政幸 |
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言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題 | siRNA, chemical modification, gene silencing, hAgo2, RISC | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ID登録 | ||||||
ID登録 | 10.15100/0000017669 | |||||
ID登録タイプ | JaLC | |||||
著者 所属 | ||||||
近畿大学大学院産業理工学研究科産業理工学専攻 | ||||||
著者 所属 | ||||||
近畿大学大学院産業理工学研究科産業理工学専攻 | ||||||
著者 所属 | ||||||
近畿大学大学院産業理工学研究科産業理工学専攻 | ||||||
著者 所属 | ||||||
近畿大学大学院産業理工学研究科産業理工学専攻 | ||||||
著者 所属 | ||||||
近畿大学産業理工学部生物環境化学科 | ||||||
著者 所属 | ||||||
近畿大学大学院産業理工学研究科産業理工学専攻 | ||||||
著者 所属 | ||||||
近畿大学産業理工学部生物環境化学科; 准教授 | ||||||
著者 所属 | ||||||
近畿大学産業理工学部生物環境化学科; 教授 | ||||||
版 | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 名前 | ||||||
出版者 | 近畿大学産業理工学部 | |||||
書誌情報 |
ja : かやのもり:近畿大学産業理工学部研究報告 en : Reports of Faculty of Humanity-Oriented Science and Engineering, Kindai University 号 24, p. 1-5, 発行日 2016-07-15 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13495801 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | For clinical applications of small interfering RNA (siRNA), chemical structure of siRNA should be optimized to be taken up into cells effectively, resistant to nuclease digestion, incorporated into RNA induced silencing complex (RISC) rapidly and correctly. They should also have minimized off-target effect and maximized turn over number. In this study, we investigated RNA interference (RNAi) efficiencies of siRNAs bearing 5’-amino-5’-deoxythymidine (T*) at 5’-end. The results showed that T* at the 5’-end of the sense strand did not interfere the processes of RISC loading complex (RLC) formation, loading to RISC or cleavage of the passenger strand by human argonaute2 (hAgo2) at all. On the other hand, T* at the 5’-end of the antisense strand gave a fatal damage to siRNA to show almost no silencing effect probably because RISC was destabilized by an electrostatic repulsion between the cationic charge of the ammonium group of T* and the cationic residues in MID domain pocket of hAgo2. These results strongly suggest that a modification of 5’-end of a sense strand with T* will suppress an off-target effect. | |||||
言語 | en | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf |