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AFLP解析によるクロマグロ, Thunnus orientalis 雄特徴的DNA断片の探索
https://kindai.repo.nii.ac.jp/records/1310
https://kindai.repo.nii.ac.jp/records/1310685c8beb-0713-4037-b74a-0f513e2a4b86
名前 / ファイル | ライセンス | アクション |
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AN00193422-20110715-0639.pdf (1.3 MB)
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Item type | ☆学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2012-08-31 | |||||
タイトル | ||||||
タイトル | AFLP解析によるクロマグロ, Thunnus orientalis 雄特徴的DNA断片の探索 | |||||
その他(別言語等)のタイトル | ||||||
その他のタイトル | Screening of the male characteristic DNA fragment using AFLP analysis in Pacific bluefin tuna, Thunnus orientalis | |||||
著者 |
阿川, 泰夫
× 阿川, 泰夫× 小宮, 貴文× 本領, 智記× 倉田, 道雄× 岡田, 貴彦× 村田, 修× 熊井, 英水× 澤田, 好史 |
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言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) | ||||||
en | ||||||
AGAWA, YASUO | ||||||
著者(英) | ||||||
en | ||||||
KOMIYA, TAKAFUMI | ||||||
著者(英) | ||||||
en | ||||||
HONRYO, TOMOKI | ||||||
著者(英) | ||||||
en | ||||||
KURATA, MICHIO | ||||||
著者(英) | ||||||
en | ||||||
OKADA, TOKIHIKO | ||||||
著者(英) | ||||||
en | ||||||
MURATA, OSAMU | ||||||
著者(英) | ||||||
en | ||||||
KUMAI, HIDEMI | ||||||
著者(英) | ||||||
en | ||||||
SAWADA, YOSHIFUMI | ||||||
著者 所属 | ||||||
近畿大学水産研究所大島実験場 | ||||||
著者 所属 | ||||||
近畿大学水産研究所大島実験場 | ||||||
著者 所属 | ||||||
近畿大学水産研究所大島実験場 | ||||||
著者 所属 | ||||||
近畿大学水産養殖種苗センター大島事業場 | ||||||
著者 所属 | ||||||
近畿大学水産養殖種苗センター大島事業場 | ||||||
著者 所属 | ||||||
近畿大学水産研究所白浜実験場 | ||||||
著者 所属 | ||||||
近畿大学水産研究所浦神実験場 | ||||||
著者 所属 | ||||||
近畿大学水産研究所大島実験場 | ||||||
著者所属(翻訳) | ||||||
Fisheries Laboratories, Kinki University, Oshima Experiment Station | ||||||
著者所属(翻訳) | ||||||
Fisheries Laboratories, Kinki University, Oshima Experiment Station | ||||||
著者所属(翻訳) | ||||||
Fisheries Laboratories, Kinki University, Oshima Experiment Station | ||||||
著者所属(翻訳) | ||||||
Oshima Fish Nursery Center | ||||||
著者所属(翻訳) | ||||||
Oshima Fish Nursery Center | ||||||
著者所属(翻訳) | ||||||
Fisheries Laboratories, Kinki University, Shirahama Experiment Station | ||||||
著者所属(翻訳) | ||||||
Fisheries Laboratories, Kinki University, Uragami Experiment Station | ||||||
著者所属(翻訳) | ||||||
Fisheries Laboratories, Kinki University, Oshima Experiment Station | ||||||
版 | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 名前 | ||||||
出版者 | 日本水産学会 | |||||
書誌情報 |
日本水産学会誌 en : Nippon Suisan Gakkaishi 巻 77, 号 4, p. 639-646, 発行日 2011-07-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00215392 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.2331/suisan.77.639 | |||||
識別番号 その他 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 10029125068 | |||||
識別番号 その他 | ||||||
内容記述タイプ | Other | |||||
内容記述 | JST.JSTAGE/suisan/77.639 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | [概要] 太平洋クロマグロ雌雄に特徴的な DNA 断片を検出する目的で AFLP 解析を行った。雌雄 4 個体ずつ計 8 個体よりゲノム DNA を調整し,64 プライマーセットで 2 次増幅した結果 1038 のバンドを検出し,内 183 が個体間で多型を示した。二次増幅を EcoRI+AGG と MseI+CAT プライマーで行った際に雄 32 個体中 29 個体,雌 32 個体中 3 個体において確認される 437 bp の断片を同定した。この断片を定量した結果,雄に於いて検出され易く,また断片の量も検出される雌より有意に(P<0.01)多かった。 [Abstract] AFLP, amplified fragment length polymorphism screening, was conducted to identify sex characteristic DNA markers of cultured Pacific bluefin tuna (PBT), Thunnus orientalis. The AFLP screening of 4 females and 4 males with 64 primer combinations produced a total of 1038 scorable bands, of which 183 were polymorphic between individuals. Male characteristic fragments were confirmed with EcoRI+AGG and MseI+CAT primers by gel image analysis, which corresponded to 437 bp in 29 of 32 males and 3 of 32 females. Fragment detail was analyzed by electropherogram analysis and DNA quantification. The 437 bp fragment was detected in 30 individuals of average DNA concentration 0.231 ng/μL; while in females, 13 individuals were detected with average 0.080 ng/μL (P<0.01). | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Copyright (c) 2011 日本水産学会, rights: 本文データは学協会の許諾に基づきJ-STAGEから複製したものである, relation: isVersionOf: https://www.jstage.jst.go.jp/article/suisan/77/4/77_4_639/_article/-char/ja/ | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal Article | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf |